Titlebook: Biophysics of the Pancreatic β-Cell; Illani Atwater,Eduardo Rojas,Bernat Soria Book 1986 The Editor(s) (if applicable) and The Author(s),

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Glucose-Evoked Changes in [K+] and [Ca2+] in the Intercellular Spaces of the Mouse Islet of Langerhanvolves the cyclic activation of K-channels.. Furthermore, it is well documented that the action potentials during the bursts result from the activation of two voltage-gated membrane channels, Ca-channels. and K-channels.

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Pharmacological Properties of the Chromaffin Cell Calcium Channelry cells which allows extracellular Ca. to penetrate them and trigger the catecholamine ejection process.” The formation accumulated since then can be summarized in the diagram of the sequence of events taking place during the secretory cycle depicted in Fig. 1.

GLIB

Calcium and Potassium Currents Recorded from Pancreatic β-Cells Under Voltage Clamp Controle secretory and electrical responses are furthermore known to be closely correlated.. From membrane potential recordings obtained with conventional intracellular electrodes and from flux measurements at least three types of currents have been postulated..

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Effects of Verapamil and Nifedipine on Glucose-Induced Electrical Activity in Pancreatic β-Cellss thought to be triggered by an increase in the cytosolic calcium concentration which appears partially related to the gating of Ca-channels, located at the plasma membrane. Experiments with Ca-channel blockers have confirmed this hypothesis: D600, Verapamil and Nifedipine cause a dose-dependent inhibition of the glucose-induced insulin release..

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(Diego Lerman. 2002, Argentina)The development of the patch-clamp technique, nearly ten years ago, allowed electrical measurements to be made under voltage-clamp conditions on small cells unsuitable for two microeletrodes voltage-clamp techniques (see[14]).

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Guatemala, an Alternation in Continuitysmitters. Examples include secretory granules from chromaffin cells,. pancreatic B-cells,. platelets. and mast cells. We have recently shown. that it is possible to obtain real-time measurements of the kinetics of secretion of vesicle contents by monitoring ATP release from stimulated medullary chromaffin cells using luciferin-luciferase.

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