Titlebook: Bioluminescence; Methods and Protocol Preston B. Rich,Christelle Douillet Book 2009Latest edition Humana Press 2009 ATP.Calcium.Colon.Expre

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High-Throughput Quantitative Bioluminescence Imaging for Assessing Tumor Burden,sophisticated questions of molecular biology by including specific promoter sequences. This chapter will describe routine methods used to support multiple investigators in our small animal imaging resource.

EWE

,Fluorescence Imaging of Tumors with “Smart” pH-Activatable Targeted Probes,argeted probes can be applied to any target molecules on the cell surface that are to be internalized after ligand binding, this imaging strategy can afford a general and powerful method to diagnose and monitor the target tumors.

CUB

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Real-Time Bioluminescence Imaging of Viral Pathogenesis,tudies of viral infection in mouse models, we and others are using noninvasive bioluminescence imaging to track viral infection, dissemination, and effects of host immune mediators on disease. In this chapter, we detail experimental protocols for bioluminescence imaging of viral infections in living mice.

Rodent

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PIN-G Reporter for Imaging and Defining Trafficking Signals in Membrane Proteins,er protein (PIN-G), containing HA, cMyc and GFP epitope, and fluorescence tags. Although originally designed for trafficking studies, pIN technology is a powerful tool applicable to almost every area of biology. Here we describe the methodologies used routinely in analyzing pIN constructs and some of their derivatives.

GRATE

Book 2009Latest editionas endured the gene- tions.Amidtheexcitement,myfatheroftentoldthestoryofhow,whenhewasachild, researchers at the Johns Hopkins University had appealed for the systematic capture of live fireflies en masse. Science had engaged the Baltimore youth in an entrepreneurial quest to jar as many lightning bu

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https://doi.org/10.1007/978-3-322-81855-3 imaging has proven difficult because of low light yield. Here we describe a procedure that combines virus-based expression of targeted aequorins with photon-counting imaging. This methodology allows real-time resolution of changes of cytosolic, mitochondrial or nuclear Ca. signals at the single-cell level.