Titlebook: Bioluminescence; Methods and Protocol Preston B. Rich,Christelle Douillet Book 2009Latest edition Humana Press 2009 ATP.Calcium.Colon.Expre

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FACT

Bioluminescence Imaging of Calcium Oscillations Inside Intracellular Organelles, imaging has proven difficult because of low light yield. Here we describe a procedure that combines virus-based expression of targeted aequorins with photon-counting imaging. This methodology allows real-time resolution of changes of cytosolic, mitochondrial or nuclear Ca. signals at the single-cell level.

古代

Novel Tools for Use in Bioluminescence Resonance Energy Transfer (BRET) Assays,e also show how they can be combined with protein complementation assays such as bimolecular fluorescence complementation (BiFC) to study three- and four-partner interactions. We also describe a BRET assay that uses SNAP-tagged proteins as a fluorescence acceptor molecule for the bioluminescent donor.

ventilate

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Günther Schuh,Hans-Peter Wiendahlative luciferases, such as those from the firefly (.) or marine copepods (.), may be more appropriate. Here we describe the protocols required to monitor colonization and clearance dynamics using bioluminescent bacteria that are .-, .-, or .-positive.

keloid

llibretto

J?rn-Henrik von Thun,Joachim Stumpfenase under the control of the same SBE promoter (SBE-lucRT mice). SBE-luc and SBE-lucRT mice can be used to study temporal, tissue-specific activation of Smad2/3-dependent signaling in living mice as well as for the identification of endogenous or synthetic modulators of this pathway.

充氣女

少量

Bioluminescent Monitoring of In Vivo Colonization and Clearance Dynamics by Light-Emitting Bacteriaative luciferases, such as those from the firefly (.) or marine copepods (.), may be more appropriate. Here we describe the protocols required to monitor colonization and clearance dynamics using bioluminescent bacteria that are .-, .-, or .-positive.

群島

Quantitative In Vivo Imaging of Non-viral-Mediated Gene Expression and RNAi-Mediated Knockdown,mary advantages of using quantitative BLI in mouse liver and muscle are the sensitivity of the assay, the speed and ease of making measurements, the precision and linearity of the dose–response curves, and the ability to conduct serial sampling of gene expression over many days or months while eliminating the need to euthanize animals.