Titlebook: Bioconjugation; Methods and Protocol Sam Massa,Nick Devoogdt Book 2019 Springer Science+Business Media, LLC, part of Springer Nature 2019 P

女歌星

VEIL

Site-Selective Enzymatic Labeling of Designed Ankyrin Repeat Proteins Using Protein Farnesyltransfe C-terminal CaaX motif at the C-terminus of a protein substrate. The addition of only four amino acid residues minimizes the influence on the native protein structure. In addition, a variety of isoprenoid analogs containing different bioorthogonal functional groups, including azides, alkynes, and al

geometrician

,Homogeneous Antibody–Drug Conjugates via Glycoengineering,ties using sodium periodate provided an aldehyde handle on the glycans for conjugation with an aminooxy-assembled payload. These two strategies both enable gsADCs with high homogeneity in their conjugation sites, payload numbers, and glycoforms, which are characterized of a single mass under mass-sp

鉆孔

Facile Site-Specific Multiconjugation Strategies in Recombinant Proteins Produced in Bacteria,, a non-IgG binding scaffold, in a specific . strain to obtain high yields of protein carrying both a thiol and an azide group. We provide straightforward protocols for strain-promoted azide–alkyne cycloaddition (SPAAC) and thiol–maleimide conjugation, and furthermore compare these conjugation chemi

熄滅

Apoptosis

構(gòu)成

https://doi.org/10.1007/978-3-658-06581-2quence called Tub-tag. Here we describe the one-step Tub-tag protein modification protocol in detail and explain its utilization to generate fluorescently labeled proteins for advanced applications in imaging and diagnostics.

思想上升

,Engineering Dual Variable Domains for the Generation of Site-Specific Antibody–Drug Conjugates,iable-domain (DVD) format. Here we provide protocols for the engineering, expression, and purification of the DVDs used for this strategy. We also provide a protocol for DVD–drug conjugation and describe methods for their biochemical characterization, including a catalytic assay to monitor conjugation efficiency.

anthropologist

人造