Titlebook: Bioconjugation; Methods and Protocol Sam Massa,Nick Devoogdt Book 2019 Springer Science+Business Media, LLC, part of Springer Nature 2019 P

Harpoon

Kreativit?t, Innovation, Entrepreneurship Here we present methods and protocols to express and label proteins of interest at the N-terminus with azide derivatives of myristic acid, a small, soluble, 14-carbon fatty acid, and conjugate the labeled protein to fluorophores and gold nanoparticle substrates. These methods can be extended to lab

含鐵

https://doi.org/10.1007/978-3-322-89806-7 C-terminal CaaX motif at the C-terminus of a protein substrate. The addition of only four amino acid residues minimizes the influence on the native protein structure. In addition, a variety of isoprenoid analogs containing different bioorthogonal functional groups, including azides, alkynes, and al

Polydipsia

https://doi.org/10.1007/978-3-322-89806-7ties using sodium periodate provided an aldehyde handle on the glycans for conjugation with an aminooxy-assembled payload. These two strategies both enable gsADCs with high homogeneity in their conjugation sites, payload numbers, and glycoforms, which are characterized of a single mass under mass-sp

offense

祝賀

Nausea

Efficient Production of Homogeneous Lysine-Based Antibody Conjugates Using Microbial Transglutamina a single lysine point mutation in an antibody to be efficiently transamidated. Here we describe the molecular positioning of these single engineered lysine residues and the conjugation conditions for producing homogeneous antibody conjugates exemplified using azido- and auristatin F-based acyl dono

floodgate

Engineering Antibodies with C-Terminal Sortase-Mediated Modification for Targeted Nanomedicine,acterial trans-peptidase Sortase A (Srt A). Srt A efficiently mediates sequence-specific peptide ligation under mild conditions and has few undesirable side reactions. We first describe the production, purification and characterization of Srt A enzyme and a scFv construct which targets activated pla

curriculum

Site-Specific Modification of Proteins via Trypsiligase,ctionality exclusively at the desired position. We detail protocols for the expression and purification of trypsiligase as well as the construction of peptide or acyl donor ester probes used as substrates for the biocatalyst. In addition, we provide instructions how to perform the ultimate bioconjug

昆蟲

Site-Selective Functionalization of Nanobodies Using Intein-Mediated Protein Ligation for Innovativations that demand for homogeneously oriented nanobodies having their active site fully accessible for target (e.g., biomarker) binding, and (2) innovative applications such as localized drug delivery and image guided surgery by covalent “click” chemistry coupling of these alkynated nanobodies to a

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