Titlebook: Bacteriophages; Methods and Protocol Martha R.J. Clokie,Andrew M. Kropinski Book 2009 Humana Press 2009 Bacteria.Microbial ecology.Microbia

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Enumeration of Bacteriophages by Double Agar Overlay Plaque AssayThe determination of the concentration of infectious phage particles is fundamental to many protocols in phage biology, genetics, and molecular biology. In this chapter the classical overlay protocol is described.

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Enumeration of Bacteriophages by the Direct Plating Plaque AssayA method is described for determination of the concentration of infectious phage particles by the direct plating plaque assay, which is simpler and faster than the double agar overlay plaque procedure outlined in the previous chapter.

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Basic Phage Electron MicroscopyNegative staining of purified viruses is the most important electron microscopical technique in virology. The principal stains are phosphotungstate and uranyl acetate, both of which have problems and advantages. Particular problems are encountered in photography, calibration of magnification, measurements, and interpretation of artifacts.

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