Titlebook: Bacteriophages; Methods and Protocol Martha R.J. Clokie,Andrew M. Kropinski Book 2009 Humana Press 2009 Bacteria.Microbial ecology.Microbia

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Enumeration of Bacteriophages Using the Small Drop Plaque Assay Systemy. Described here is a drop plaque assay, which, being simpler, faster and more efficient than either the classical overlay or direct plating methods, enhances efficiency in processing large numbers of samples.

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Handbuch Historisches MauerwerkMost bacterial cells carry prophage genomes either integrated into the host DNA or present as repressed plasmids. Methods are described for the induction of prophages using Mitomycin C, and for the isolation of prophage-cured bacterial cell lines.

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Hochtemperaturfestigkeit und -verformung,The determination of the concentration of infectious phage particles is fundamental to many protocols in phage biology, genetics, and molecular biology. In this chapter the classical overlay protocol is described.

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https://doi.org/10.1007/978-3-8348-9907-1A method is described for determination of the concentration of infectious phage particles by the direct plating plaque assay, which is simpler and faster than the double agar overlay plaque procedure outlined in the previous chapter.

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Zyklische Festigkeit und Verformung,Negative staining of purified viruses is the most important electron microscopical technique in virology. The principal stains are phosphotungstate and uranyl acetate, both of which have problems and advantages. Particular problems are encountered in photography, calibration of magnification, measurements, and interpretation of artifacts.