Titlebook: Bacterial Protein Secretion Systems; Methods and Protocol Laure Journet,Eric Cascales Book 2017 Springer Science+Business Media LLC 2017 Fr

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Alcove

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BIAS

,Protein–Protein Interactions: Co-Immunoprecipitation,eraction events in vivo. Co-IP experiments can identify proteins via direct or indirect interactions or in a protein complex. Here, we use . type VI secretion system (T6SS) sheath components TssB-TssC. interaction as an example to describe the principle, procedure, and experimental problems of co-IP.

CAMEO

Book 2017s on troubleshooting and avoiding known pitfalls..Authoritative and cutting-edge, .Bacterial Protein Secretion Systems: Methods and Protocol .aims to provide techniques that are not restricted to the study of secretion systems but arealso of specific interest for any researcher interested on multi-protein complexes of the bacterial cell envelope..

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Book 2017and localizing the different subunits, defining interactions within subunits, monitoring conformational changes, purifying and imaging of large complexes, defining the assembly pathway by fluorescence microscopy and the role of energy during assembly and/or secretion, identifying secreted effectors

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occurrence

Identification of Protein Secretion Systems in Bacterial Genomes Using MacSyFinder,t checks whether the genetic content and organization of clusters satisfy the constraints of the model. TXSScan models can be customized to search for variants of known systems. The models can also be built from scratch to identify novel systems. In this chapter, we describe a complete pipeline of a

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