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Titlebook: Bacterial Protein Secretion Systems; Methods and Protocol Laure Journet,Eric Cascales Book 2017 Springer Science+Business Media LLC 2017 Fr

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發(fā)表于 2025-3-21 17:55:54 | 只看該作者 |倒序瀏覽 |閱讀模式
期刊全稱Bacterial Protein Secretion Systems
期刊簡稱Methods and Protocol
影響因子2023Laure Journet,Eric Cascales
視頻videohttp://file.papertrans.cn/181/180315/180315.mp4
發(fā)行地址Includes cutting-edge methods and protocols.Provides step-by-step detail essential for reproducible results.Contains key notes and implementation advice from the experts
學科分類Methods in Molecular Biology
圖書封面Titlebook: Bacterial Protein Secretion Systems; Methods and Protocol Laure Journet,Eric Cascales Book 2017 Springer Science+Business Media LLC 2017 Fr
影響因子.This volume details protocols that cover the broad arsenal of techniques used to study a secretion system from A to Z. Chapters focus on identifying and localizing the different subunits, defining interactions within subunits, monitoring conformational changes, purifying and imaging of large complexes, defining the assembly pathway by fluorescence microscopy and the role of energy during assembly and/or secretion, identifying secreted effectors as well as reporters to follow effector transport. Written in the highly successful .Methods in Molecular Biology .series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls..Authoritative and cutting-edge, .Bacterial Protein Secretion Systems: Methods and Protocol .aims to provide techniques that are not restricted to the study of secretion systems but arealso of specific interest for any researcher interested on multi-protein complexes of the bacterial cell envelope..
Pindex Book 2017
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https://doi.org/10.1007/978-1-4939-7033-9Fractionation; Lipoproteins; Peptidoglycan; Protease; sub-cellular localisation
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Birger P. Priddat,Christoph Meinekemethods exist for predicting surface-exposed lipoproteins, and therefore lipoprotein topology must be experimentally tested. This chapter describes three distinct but complementary methods for the detection of surface-exposed proteins: cell surface protein labeling, accessibility to extracellular protease and antibodies.
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D. Michael Albrecht,Armin T?pferuped into three fundamentally different approaches: signal-based, global-property-based and homology-based prediction. In this chapter, the strengths and drawbacks of each of these approaches is described through many examples of methods that predict secretion, integration into membranes, or subcell
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