| 期刊全稱(chēng) | Bacterial Protein Secretion Systems | | 期刊簡(jiǎn)稱(chēng) | Methods and Protocol | | 影響因子2023 | Laure Journet,Eric Cascales | | 視頻video | http://file.papertrans.cn/181/180315/180315.mp4 | | 發(fā)行地址 | Includes cutting-edge methods and protocols.Provides step-by-step detail essential for reproducible results.Contains key notes and implementation advice from the experts | | 學(xué)科分類(lèi) | Methods in Molecular Biology | | 圖書(shū)封面 |  | | 影響因子 | .This volume details protocols that cover the broad arsenal of techniques used to study a secretion system from A to Z. Chapters focus on identifying and localizing the different subunits, defining interactions within subunits, monitoring conformational changes, purifying and imaging of large complexes, defining the assembly pathway by fluorescence microscopy and the role of energy during assembly and/or secretion, identifying secreted effectors as well as reporters to follow effector transport. Written in the highly successful .Methods in Molecular Biology .series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls..Authoritative and cutting-edge, .Bacterial Protein Secretion Systems: Methods and Protocol .aims to provide techniques that are not restricted to the study of secretion systems but arealso of specific interest for any researcher interested on multi-protein complexes of the bacterial cell envelope.. | | Pindex | Book 2017 |
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