Titlebook: Bacterial Artificial Chromosomes; Volume 1: Library Co Shaying Zhao,Marvin Stodolsky Book 2004 Humana Press 2004 BAC.Chromosom.DNA.DNA sequ

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https://doi.org/10.1007/978-1-4020-7964-1s. The approach chosen by the International Human Genome Sequencing Consortium is a hierarchical mapping and sequencing strategy similar to that described for other large genomes, including ., and . (.–.). A physical map of the human genome has been assembled on the basis of “fingerprints” generated

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Construction of Small Genome BAC Library for Functional and Genomic Applications,nic; and, second, a broad spectrum of genetic techniques for the particular bacteria being studied must be available. For other bacteria, such as the syphilis-causing spirochete, ., genetic studies are highly limited because the bacterium cannot be continuously grown in the laboratory, because of it

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Exploring Transformation-Associated Recombination Cloning for Selective Isolation of Genomic RegionBAC) cloning systems (.,.). These techniques have made the isolation of large, random DNA fragments possible, thereby greatly simplifying the physical mapping of chromosomes. However, isolation of entire genes and specific chromosomal regions for functional studies remained a laborious process becau

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Purification of BAC DNA, DNA from different species. These BAC libraries are constructed by insertion of DNA fragments from different species into a vector, which can be replicated in a bacterial host. Choosing . as the model host has many advantages: rapid growth of the host, high stability of the DNA fragment when inside

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Hybridization-Based Selection of BAC Clones,derived from target genomic regions is essential for detailed analysis such as sequencing. Since its introduction in 1992, the bacterial artificial chromosome (BAC) library system has been widely employed as a standard cloning system for mapping and sequencing the genomes of human and model organism

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BAC Mapping Using Fluorescence , Hybridization,rstanding disease, development and evolution. To accomplish this goal and a broad spectrum of applications requires integration of genome sequence information to genetic markers (expressed sequence tag/cDNA/gene transcripts content) and to reagents that can be seen through a microscope and linked to

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