Titlebook: Bacterial Artificial Chromosomes; Volume 1: Library Co Shaying Zhao,Marvin Stodolsky Book 2004 Humana Press 2004 BAC.Chromosom.DNA.DNA sequ

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Anatomy and Function of the Hand et al. (.) for use in the construction of high-resolution physical maps from low-copynumber, large-insert clones. The procedure is robust and allows for the recovery of clone insert size information. Initially used to construct sequence tag site (STS)-based contigs (.), the methodology has also bee

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Handbook of Upper Extremity Examinationr fragments; end sequencing these smaller fragments; and from the overlapping sequences of the randomly generated fragments, reassembling the initial target sequence. Although this random strategy initially was described more than two decades ago (.–.), only a few years following the original report

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Sexual Minority Groups and Urban Healthn have been limited to purification of biologically propagated M13 or plasmid-based templates, or in vitro amplification of such templates by polymerase chain reaction (PCR). In this chapter, we describe a protocol for a new approach to template generation: rolling circle amplification (RCA). We hav

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Mark R. Montgomery,Alex C. Ezeh stages of bacterial artificial chromosome (BAC) sequencing, allowing the user to expand a finishing repertoire of custom oligonucleotide sequencing, alternative chemistry sequencing, polymerase chain reaction (PCR), as well as other standard tools for acquiring high-quality data in low-coverage or

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Tree Inventory and Systematic Management,in a typical amplification, the primers should hybridize only to the target sequence. However, there is a relatively narrow range of conditions (temperature, concentrations of ions and denaturing agents) for specific annealing of an oligonucleotide to its complementary target, and often it does not

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Tree Roots versus Sidewalks and Sewers, methods for obtaining position-specific sequence information along the insert DNA. Because substantial quantities of high-quality clone DNA can be easily prepared from BACs and PACs, they are also potentially valuable reagents for direct use in biologic experiments that seek to functionally evaluat

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Interpersonally Comparable Utility,n a BAC or P1 vector have been used to sequence either end of a genomic insert to facilitate selection of minimally overlapping clones for analyzing large chromosomal regions (.). The entire insert in a BAC or P1-derived artificial chromosome (PAC) clone, however, is usually sequenced using shotgun