Titlebook: Axonal Transport; Methods and Protocol Alessio Vagnoni Book 2022 The Editor(s) (if applicable) and The Author(s), under exclusive license t

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心胸狹窄

Cancer Drug Discovery and Developmentctivity and transport because?its defined synaptic circuits can be built in-vitro. Advantages include precise pre- and postsynaptic manipulation, and high-resolution imaging of axonal transport. Here, we describe methodologies for the quantitative analysis of axonal transport in . sensory neurons.

易怒

Hypoxia and Reactive Oxygen Speciesed out in the context of a developed nerve containing both axons and glia. Here we outline how to image axonal transport ex vivo in sciatic and optic nerves, and the fimbria of the fornix. These methods could be altered to image other fluorescently labeled molecules, as well as different mechanisms of intracellular transport.

VICT

Scintigraphy

The Giant Axon of the Squid: A Simple System for Axonal Transport Studiesar transport. In this protocol we describe the method used for dissecting the axon to preserve its viability in vitro, and the technique for injecting exogenous materials into the living axon. Now that the squid genome is emerging, and the CRISPR/cas9 system has been successfully applied to knock-ou

DAMN

Gudgeon

Live Imaging of RNA Transport and Translation in , Retinal Axonsdge of the molecular mechanisms underlying axonal mRNA translation has rapidly expanded. However, tools to study mRNA localization and translation in real time with high spatial precision were not available until recently. Here, we present a live imaging approach to examine axonal mRNA trafficking a

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Imaging Axonal Transport in Ex Vivo Central and Peripheral Nerveser to remain healthy. This process, known as axonal transport, can be imaged in real time through epifluorescent imaging of fluorescently labeled proteins, organelles, and other cargoes. While this is most conveniently done in primary neuronal cultures, it is more physiologically relevant when carri

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