Titlebook: Axonal Transport; Methods and Protocol Alessio Vagnoni Book 2022 The Editor(s) (if applicable) and The Author(s), under exclusive license t

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https://doi.org/10.1007/978-1-4614-7542-2s useful for systematically comparing the localization of different kinesins in the same cell type and for identifying cargo proteins that reside in vesicles moved by a specific kinesin family member. While we describe the assay in cultured hippocampal neurons, we expect it to be easily transferable to other eukaryotic cell types.

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In Vivo Imaging of Axonal Organelle Transport in the Mouse Brainnto thalamic nuclei of mice, thereby transducing neurons that project to the surface of the brain. Axonal projections and trafficking of organelles can be imaged with a 2-photon microscope through a chronically implanted window in the mouse skull in anesthetized as well as awake mice.

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Methods and Applications of Campenot Trichamber Neuronal Cultures for the Study of Neuroinvasive Virn through the chambers, and alpha herpesvirus infection, intracellular trafficking, and cell-cell spread via axons. (2) Using correlative fluorescence microscopy and cryo electron tomography to investigate the ultrastructure of virus particles trafficking in axons.

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1064-3745 ation advice from the experts.This volume covers a wide range of model systems from invertebrate to humans and .in vitro?.and?.in vivo.. ?Chapters detail methods on squid, aplysia, xenopus, mouse in vivo, ex vivo, rodent primary neurons, human-derived neurons, zebrafish, drosophila in vivo, primary

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Nicole D. Fer,Annamaria Rapisarda PhD exogenous materials into the living axon. Now that the squid genome is emerging, and the CRISPR/cas9 system has been successfully applied to knock-out squid genes, the giant axon will resume its place in the scientific pantheon of powerful experimental systems in which to address biological questions pertaining to all eukaryotes.

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The Giant Axon of the Squid: A Simple System for Axonal Transport Studies exogenous materials into the living axon. Now that the squid genome is emerging, and the CRISPR/cas9 system has been successfully applied to knock-out squid genes, the giant axon will resume its place in the scientific pantheon of powerful experimental systems in which to address biological questions pertaining to all eukaryotes.