Titlebook: Artificial Nucleases; Marina A. Zenkova Book 2004 Springer-Verlag Berlin Heidelberg 2004 Catalysis.Cleavage.Conjugates.DNA.Enzyme mimics.N

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Book 2004nucleic acids and processing RNA; DNA-cleaving antibiotics and cytotoxic ribonucleases have demonstrated utility as chemotherapeutic agents. The second class, artificial nucleases, are rationally designed to imitate the active centers of natural enzymes by simple structures possessing minimal sets o

Suggestions

Cleavage of RNA by Imidazole,les. This simplicity appears to be an advantage when artificial RNases are used in design of therapeutics for controlling gene expression or as components of synthetic conjugates. These conjugates are targeted to specific RNA molecules by oligonucleotides, and unwanted interactions of their componen

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Allosterically Controlled Ribozymes as Artificial Ribonucleases,fic sequences of interest, as therapeutic agents and as specific regulators of gene expression (Cech 1988; Yu et al. 1995; Bertrand et al. 1997; Birikh et al. 1997; Kawasaki et al. 1998; Lan et al. 1998; Plehn-Dujowich and Altman 1998; Kuwabara et al. 1998a,b, 1999; Koseki et al. 1999; Warashina et

案發(fā)地點(diǎn)

Small Ribonuclease Mimics,8; Kozlowski et al. 1999; Kimura 2000). For nucleic acids cleaving enzymes it is suggested that hydrolysis of p-o bonds passes via acid-base catalysis (delCardayre and Raines 1995). In contrast to hydrolysis of c-o bonds, the p-o bonds are very rarely cleaved via nucleophilic catalysis (Gottlin et a

Munificent

Copper Containing Nuclease Mimics: Synthetic Models and Biochemical Applications,is proposed to have a trinuclear Zn. coordination (Shishido and Habuka 1986); . endonuclease displays Mg.-dependent nucleolytic activity, where metal ion is used to reduce the activation energy of P-O bond fission (Miller and Krause 1996).

FRET

DNA and RNA Cleavage Mediated by Phenanthroline-Cuprous Oligonucleotides: From Properties to Applic footprinting experiments to analyze the structure of protein-nucleic acid complexes. These cleavage reagents have also been tethered to larger molecules such as oligonucleotides, proteins, and intercalating agents, which confer additional specificity. This review will focus on the 1,1O-phenanthroli

PLUMP

Stress

RNA-Cleaving Oligonucleotide-Peptide Conjugates,pable of hydrolyzing RNA. The basic requirements showed by any artificial ribonucleases are high efficiency and specificity of RNA cleavage. The efficiency of hydrolysis is determined by the catalytic properties of active groups, and the specificity is determined by the affinity of compounds for certain RNA sites.

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