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Titlebook: Apoptosis Techniques and Protocols; Andréa C. LeBlanc Book 2002Latest edition Humana Press 2002

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期刊全稱(chēng)Apoptosis Techniques and Protocols
影響因子2023Andréa C. LeBlanc
視頻videohttp://file.papertrans.cn/160/159002/159002.mp4
發(fā)行地址Includes supplementary material:
學(xué)科分類(lèi)Neuromethods
圖書(shū)封面Titlebook: Apoptosis Techniques and Protocols;  Andréa C. LeBlanc Book 2002Latest edition Humana Press 2002
影響因子The study of the apoptotic process has grown exponentially since the publication of the first edition of Apoptosis Techniques and Protocols for the Neuromethods series in 1997. In this greatly updated second edition, seasoned experts describe in step-by-step detail their best state-of-the-art techniques for studying neuronal cell death. These readily reproducible methods solve a wide variety of research problems, including the detection of the key proteins involved in neuronal apoptosis (Bax protein, cytochrome-c, and caspases), the direct assessment of the role of pro-apoptotic proteins in neurons by viral infections and microinjections, and the detection of proapoptotic proteins in situ. There are also hands-on methods for the study of apoptosis mechanisms in neuronal compartments, for studying synaptosis, and for establishing gene expression profiles in neurodegenerative brain tissues by using DNA microarrays. Written by investigators who have used the techniques extensively, each protocolincludes tips on avoiding pitfalls, notes on the method‘s advantages and disadvantages, and a critical survey of the literature. .Cutting-edge and highly practical, Apoptosis Techniques and Pro
Pindex Book 2002Latest edition
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Monitoring Caspases in Neuronal Cell Death,ke the proper connections with their postsynaptic targets die naturally during development due to the lack of sufficient trophic support (.). Cell demise is operated in a wellordered fashion and culminates in the activation of a network of specific proteases, the caspases that execute the death prog
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Designing Microarray Experiments for Neurobiology,a powerful method for estimating the behavior of 10,000 genes simultaneously. But how often does it work well? Because of the rapid adoption of this young technology, we have to anticipate that one of the consequences will be the occasional publication of incorrect results. Some microarray data are
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Analysis of Gene Expression by Nylon Membrane cDNA Arrays,g this technology to an issue of potentially high relevance to current research in Alzheimer’s Disease (AD). The AD field is in strong need of biomarkers suitable for the diagnosis of AD prior to symptomology. Amyloid-beta (Aβ) deposition in the brain is a hallmark of AD, and amino acid racemization
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Glyceraldehyde-3-phosphate Dehydrogenase as a Target for Antiapoptotic Drugs,ole in glycolysis. Indeed, the most frequent reference to GAPDH in recent scientific literature is as the “housekeeping” gene used to standardize Northern blots. A careful examination of the literature of the last 25 years, however, reveals a number of novel actions of GAPDH beyond a role in glycoly
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In Situ Detection of Apoptotic Neurons,type of non-necrotic, regulated cell death observed in the nervous system, it is by far the most extensively investigated (.,.). Studies in a variety of species, cell types, and experimental paradigms have led to a better understanding of the molecular pathways that regulate apoptosis and recognitio
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