Titlebook: Antibody Phage Display; Methods and Protocol Philippa M. O’Brien,Robert Aitken Book 20021st edition Humana Press 2002

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Philippa M. O’Brien,Robert AitkenIncludes supplementary material:

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,Elektromagnetische Ausgleichsvorg?nge, to mimic as closely as possible the desired in vivo activity of the new drug. New library methodologies offer many alternative routes that are at least as powerful as traditional approaches by combining the generation of billions of components with a fast screening or selection procedure to identif

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,Der station?re elektrische Strom, surface of the filamentous phage particle, have been widely used for the isolation of antibodies (Abs) with specificity for haptens, foreign antigens (Ags), and self Ags. Immune Fab libraries, in which lymphoid tissue from individuals who, perhaps because of disease, have mounted an immune response

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Karl Küpfmüller,Gerhard Kohn (o. Professor)unology. This can be explained by the lack of appropriate myeloma lines or transforming viruses for species outside this select group and the instability of heterohydridoma cell lines generated with, for example, murine myeloma lines (.). The advent of Ab phage-display technology offers a solution t

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https://doi.org/10.1007/978-3-662-36777-3ge panels of antigens (Ags). Na?ve libraries have been generated by tapping the natural primary (unselected) immune repertoire via cloning of Abs that recognize a variety of Ags (.,.). The rearranged V genes were amplified with the polymerase chain reaction (PCR) from B-cell mRNAs encoding immunoglo