Titlebook: Animal Cell Biotechnology; Methods and Protocol Nigel Jenkins Book 19991st edition Springer Science+Business Media New York 1999

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Monitoring Animal Cell Growth and Productivity by Flow Cytometryanalyzed to provide information on the relationship between them. Fluorescently labeled cells can give quantitative data on specific target molecules and their distribution in the cell population. Moreover, subpopulations of cells with specific attributes can be discriminated and physically sorted f

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Nuclear Magnetic Resonance Methods of Monitoring Cell Metabolismsmall organic molecules. The advent of Fourier transform NMR, coupled with the development of superconducting magnets with higher field strengths, opened the technique to a variety of biological and clinical applications. NMR is now a proven technique for monitoring metabolism in diverse systems—iso

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Culturing Animal Cells in Fluidized Bed Reactorsestablished technique and has been successfully used from laboratory to production scale in stirred tank reactors. However, as the loading of the microcarriers is raised to increase cell density further, adequate mixing of the culture environment by stirring becomes problematic. To achieve high load

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GPI-Anchored Fusion Proteinse group of proteins is uniquely anchored to the plasma membrane by the covalent attachment of a complex phospolipid anchor to the carboxyl terminus of the protein. This glycosylphosphatidyl inositol (GPI) anchor consists of a hydrophobic phospholipid, phosphatidylinositol, which attaches the carboxy

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Cytogenetic Characterization of Recombinant Cellsression vector have been integrated, cellular resistance to methotrexate (MTX), a folate analog, is mediated. MTX can therefore be used to select for subclones that have amplified the transfected plasmid sequences to very high copy numbers.

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DNA Fingerprinting and Characterization of Animal Cellsact, turned out to be subclones of the HeLa cell line. Thus, it is important that all workers using cell lines be aware of this problem and be able to take steps to avoid the use of bogus cell lines This chapter is intended to give the reader an overview of some of the more popular DNA identificatio

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