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Titlebook: Animal Cell Biotechnology; Methods and Protocol Nigel Jenkins Book 19991st edition Springer Science+Business Media New York 1999

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期刊全稱Animal Cell Biotechnology
期刊簡稱Methods and Protocol
影響因子2023Nigel Jenkins
視頻videohttp://file.papertrans.cn/158/157553/157553.mp4
學科分類Methods in Biotechnology
圖書封面Titlebook: Animal Cell Biotechnology; Methods and Protocol Nigel Jenkins Book 19991st edition Springer Science+Business Media New York 1999
影響因子Scientists with long-refined expertise describe cutting-edge techniques for the production of therapeutic proteins and vaccines. Capturing the major advances that have occurred in both the science and the technology of these biopharmaceuticals, this important book covers the powerful new techniques used in genetically manipulatinganimal cells, optimizing their growth in defined media (particularly at large-scale), avoiding contamination, and in the harvesting and analysis of cell products. Topics include basic culture facilities and methods; molecular methods for gene transfection, cell immortalization and cell fusion; and techniques for the study of cell growth, viability, metabolism, and productivity. Animal Cell Biotechnology constitutes a comprehensive manual of state-of-the-art techniques for setting up a cell culture laboratory, maintaining cell lines, and optimizing critical parameters for cell culture. .
Pindex Book 19991st edition
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書目名稱Animal Cell Biotechnology影響因子(影響力)




書目名稱Animal Cell Biotechnology影響因子(影響力)學科排名




書目名稱Animal Cell Biotechnology網(wǎng)絡公開度




書目名稱Animal Cell Biotechnology網(wǎng)絡公開度學科排名




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書目名稱Animal Cell Biotechnology被引頻次學科排名




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Adaptation of Mammalian Cells to Growth in Serum-Free Medialtivation in suspension culture, without serum, and at high cell densities. Such phenotypes are useful for the production of recombinant proteins and monoclonal antibodies for therapeutic or diagnostic applications. Emphasis will be placed on Chinese hamster ovary (CHO) cells due to their widespread
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Optimizing Gene Expression in Mammalian Cellsalian cells are essential to perform such modifications correctly, and efficient methods have been established to transduce or transfect them, based on either viral or synthetic vectors, or combinations of both. Several parameters affect the efficiency of expression of the transgene, such as the nat
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Immortalization Strategies for Mammalian Cellsescence. However, when the control mechanisms which limit life span are modified cells can become immortalized i.e. have an unlimited growth potential (.). One such way of subverting these control mechanisms is to express an immortalizing oncogene in normal cells. These proteins override the normal
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Cell Bank Preparation and Characterizationerties of a cell line or of a product derived from it, it is, therefore, necessary to cryopreserve and characterize a reference stock of cells. Thereafter, throughout a research, development, or manufacturing program cell cultures are discarded after a defined passage interval and the cryopreserved
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Cell Counting and Viability Measurementst method is by cell counting at regular intervals. In routine cultures this would be performed once a day, which corresponds to the approximate doubling time of mammalian cells during the exponential growth phase. This would establish an overall growth profile of a culture. More frequent counts woul
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