| 期刊全稱 | Animal Cell Biotechnology | | 期刊簡稱 | Methods and Protocol | | 影響因子2023 | Ralf P?rtner | | 視頻video | http://file.papertrans.cn/158/157551/157551.mp4 | | 發(fā)行地址 | Includes cutting-edge methods and protocols.Provides step-by-step detail essential for reproducible results.Contains key notes and implementation advice from the experts | | 學科分類 | Methods in Molecular Biology | | 圖書封面 |  | | 影響因子 | .Animal Cell Biotechnology: Methods and Protocols, Third Edition. constitutes a comprehensive manual of state-of-the-art and new techniques for setting up mammalian cell lines for production of biopharmaceuticals, and for optimizing critical parameters for cell culture from lab to final production. The volume is divided into five parts that reflect the processes required for different stages of production. In Part I, basic techniques for establishment of production cell lines are addressed, especially high-throughput synchronization, insect cell lines, transient gene and protein expression, DNA Profiling and Characterisation. Part II addresses tools for process and medium optimization as well as microcarrier technology while Part III covers monitoring of cell growth, viability and apoptosis, metabolic flux estimation, quenching methods as well as NMR-based techniques. Part IV details cultivation techniques, and Part V describes special applications, including vaccine production, baculovirus protein expression, chromatographic techniques for downstream as well as membrane techniques for virus separation. Written in the successful .Methods in Molecular Biology. series format, chapter | | Pindex | Book 2014Latest edition |
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