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Titlebook: Animal Cell Biotechnology; Methods and Protocol Ralf P?rtner Book 2014Latest edition Springer Science+Business Media, LLC 2014 DNA profilin

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發(fā)表于 2025-3-21 18:34:57 | 只看該作者 |倒序瀏覽 |閱讀模式
期刊全稱Animal Cell Biotechnology
期刊簡稱Methods and Protocol
影響因子2023Ralf P?rtner
視頻videohttp://file.papertrans.cn/158/157551/157551.mp4
發(fā)行地址Includes cutting-edge methods and protocols.Provides step-by-step detail essential for reproducible results.Contains key notes and implementation advice from the experts
學(xué)科分類Methods in Molecular Biology
圖書封面Titlebook: Animal Cell Biotechnology; Methods and Protocol Ralf P?rtner Book 2014Latest edition Springer Science+Business Media, LLC 2014 DNA profilin
影響因子.Animal Cell Biotechnology: Methods and Protocols, Third Edition. constitutes a comprehensive manual of state-of-the-art and new techniques for setting up mammalian cell lines for production of biopharmaceuticals, and for optimizing critical parameters for cell culture from lab to final production. The volume is divided into five parts that reflect the processes required for different stages of production. In Part I, basic techniques for establishment of production cell lines are addressed, especially high-throughput synchronization, insect cell lines, transient gene and protein expression, DNA Profiling and Characterisation. Part II addresses tools for process and medium optimization as well as microcarrier technology while Part III covers monitoring of cell growth, viability and apoptosis, metabolic flux estimation, quenching methods as well as NMR-based techniques. Part IV details cultivation techniques, and Part V describes special applications, including vaccine production, baculovirus protein expression, chromatographic techniques for downstream as well as membrane techniques for virus separation. Written in the successful .Methods in Molecular Biology. series format, chapter
Pindex Book 2014Latest edition
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書目名稱Animal Cell Biotechnology影響因子(影響力)




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書目名稱Animal Cell Biotechnology網(wǎng)絡(luò)公開度學(xué)科排名




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Ralf P?rtnerIncludes cutting-edge methods and protocols.Provides step-by-step detail essential for reproducible results.Contains key notes and implementation advice from the experts
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https://doi.org/10.1007/978-1-349-22299-5edge of cell requirements increased steadily. In the beginning, animal-sourced components were the key to growth. Nowadays state-of-the-art media do not contain any animal or naturally sourced components. The compositions are based on scientific awareness of the needs of the cells. The result is hig
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https://doi.org/10.1007/978-1-349-22299-5epletion of important medium components and results in improved culture longevity and high cell and product yields. To take maximum advantage of these effects, it is important to optimize the fed-batch process for each application. In this chapter, a simple strategy for fed-batch optimization is des
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