Titlebook: Alzheimer’s Disease; Methods and Protocol Jerold Chun Book 2023 The Editor(s) (if applicable) and The Author(s), under exclusive license to

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Protocol for the Systematic Fixation, Circuit-Based Sampling, and Qualitative and Quantitative Neurocing techniques, it has become possible to use human brain tissue to study, in great detail, the structural, molecular, and even functional underpinnings of human brain disease. In the century following the first description of Alzheimer’s disease (AD), numerous technological advances applied to hum

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Microglia in Human Postmortem Brain Samples: Quantitative Ultrastructural Analysis of Scanning Electd procedure is provided to improve the ultrastructural quality of the samples, using aldehyde fixatives followed by immunoperoxidase staining of allograft inflammatory factor 1 (AIF1, also known as IBA1), a marker of myeloid cells, and cluster of differentiation . (CD68), a marker of phagolysosomal

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Three-Dimensional Biohybrid StarPEG–Heparin Hydrogel Cultures for Modeling Human Neuronal Developmen-based 3D cell culture system, in a hydrogel, that can be used to study human neuronal development and Alzheimer’s disease (AD) pathology. A 3D cell culture system can mimic the in vivo cellular environment better than a 2D format, in which these cells exhibit neural network formation, electrophysio