Titlebook: Alternative Splicing; Methods and Protocol Peter Scheiffele,Oriane Mauger Book 2022 The Editor(s) (if applicable) and The Author(s), under

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,Probing Liquid–Liquid Phase Separation of RNA-Binding Proteins In Vitro and In Vivo,cellular content and compartmentalize biochemical reactions, in particular many processes involving RNA. This protocol is aimed at readers new to the LLPS field who want to test their protein or cellular structure of interest. We describe the basic principles of liquid–liquid phase separation, and o

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Analysis of Oligonucleotide Biodistribution and Metabolization in Experimental Animals, or bone marrow cells is possible by chemical ligation PCR. This method works independently of chemical modifications of the oligonucleotide and/or its conjugations to lipid or peptide moieties. Moreover, metabolization intermediates can be detected by mass spectrometry. Together with a readout assa

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Peter Scheiffele,Oriane MaugerIncludes cutting-edge techniques.Provides step-by-step detail essential for reproducible results.Contains key implementation advice from the experts

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https://doi.org/10.1007/978-1-0716-2521-7Gene regulation; Proteome diversification; Transcript dynamics; Therapeutic strategies; High-resolution

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In Situ Imaging of mRNA Splicing Variants by SpliceRCA,ngle-molecule resolution, discriminating splicing isoforms with single-base precision as well as analyzing the subcellular localization of transcripts. With this technology, it is possible to study cell heterogeneity of gene expression, potentially helping to decipher rich diversity in posttranscriptional function and assist clinical monitoring.

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https://doi.org/10.1007/978-3-319-10536-9 of the genome but also enables complex mechanisms for the post-transcriptional control of gene expression. Regulation of alternative splicing entails a combinatorial interplay between an abundance of .-acting splicing factors, .-acting regulatory sequence elements and their concerted effects on the

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Finance and Capital Markets Serieseutics that can modulate splicing has been dominated by antisense oligonucleotides (ASOs) and small molecule compounds, with each platform achieving remarkably effective results in the clinic. The success of RNA-targeting drugs has led to the exploration of new strategies to expand the repertoire of

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