Titlebook: Alternative Splicing; Methods and Protocol Peter Scheiffele,Oriane Mauger Book 2022 The Editor(s) (if applicable) and The Author(s), under

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,BaseScope? Approach to Visualize Alternative Splice Variants in Tissue, relative to e37b-. and both exons share 60% of their sequence. By using BaseScope?, we were able to discover that e37a-. is expressed in excitatory pyramidal neurons of hippocampus and cortex, as well as motor neurons of the ventral horn of the spinal cord.

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Book 2022tion of the alternative splicing mechanism and its targeting for therapeutic strategies, the book continues with techniques for analyzing alternative splicing profiles in complex biological systems, visualizing and localizing alternative spliced transcripts with cellular and sub-cellular resolution,

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https://doi.org/10.1007/978-94-017-1251-4characterization of RNA isoforms and led to the perpetual incrementation of gene expression diversity. Here, we describe a high throughput approach to assess in-depth the splicing regulation of target gene(s) using the third-generation sequencing (TGS) technologies.

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https://doi.org/10.1007/978-1-4419-9713-5 reporter system that enables the visualization of alternative splicing patterns by microscopy at single-cell resolution in live animals. We present this reporter system in the context of the model nematode ..

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