Titlebook: Alpha-Synuclein; Methods and Protocol Tim Bartels Book 2019 Springer Science+Business Media, LLC, part of Springer Nature 2019 endocytosis.

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Recombinant Expression and Purification of ,-Acetylated Alpha-Synuclein,n systems such as . would not normally be Nt-acetylated as these cells lack the appropriate .-α-terminal acetylation complex. Here we describe a simple protocol that allows the recombinant expression and purification of Nt-acetylated alpha-synuclein (aS) from ..

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Probing Alpha-Synuclein Conformations by Electron Paramagnetic Resonance (EPR) Spectroscopy, interactions of intrinsically disordered proteins as alpha-synuclein..Here we describe all steps required for a corresponding study: the spin labeling procedure, sample preparation, spectroscopic experimental procedure, and data analysis.

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https://doi.org/10.1007/978-3-030-59169-4Here we describe a method using mass spectrometry to characterize and quantify immuno-enriched α-synuclein forms from biochemically fractionated brain tissue.

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Kinetic Measurements of Endocytosis and Exocytosis in Cultured Neurons,ws us to study exocytosis and endocytosis in neurons. When neurons are transfected with this protein we can measure the rate of vesicles fusing and internalizing from the membrane using live total internal reflection fluorescence (TIRF) imaging. Here, we describe transfection, culturing, and imaging