Titlebook: Alpha-Synuclein; Methods and Protocol Tim Bartels Book 2019 Springer Science+Business Media, LLC, part of Springer Nature 2019 endocytosis.

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https://doi.org/10.1007/978-3-030-59169-4in dopaminergic and other types of neurons. Because α-synuclein accumulation leads to a toxic gain of function, its ectopic expression in . has been a useful in vivo model for testing modifiers of its toxicity. This chapter describes four assays: the rapid iterative negative geotaxis, rough eye phen

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neurologist

R. Hamid,Y. Khairullah,A. R. Khalimin the immediate vicinity of αSyn in living rat cortical neurons. To isolate these interactions, we transduced primary cortical neurons with a lentivirus encoding APEX2 tagged to the C-terminus of alpha-synuclein (αSyn) and under the control of a synapsin promoter. Neural protein lysates were then i

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A. I. Yilmaz,U. Albostan,A. Caner,O. Kurc interactions of intrinsically disordered proteins as alpha-synuclein..Here we describe all steps required for a corresponding study: the spin labeling procedure, sample preparation, spectroscopic experimental procedure, and data analysis.

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Methods in Molecular Biologyhttp://image.papertrans.cn/a/image/153908.jpg

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Developments in Injection Moulding—3 . in diseased brain has been a challenge in the field for some time. Here we describe a method, the alpha-synuclein proximity ligation assay (AS-PLA), to detect alpha-synuclein oligomers in paraffin-embedded brain sections. Using AS-PLA previously unobserved alpha-synuclein oligomeric pathology is revealed.

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A. I. Yilmaz,U. Albostan,A. Caner,O. Kurc interactions of intrinsically disordered proteins as alpha-synuclein..Here we describe all steps required for a corresponding study: the spin labeling procedure, sample preparation, spectroscopic experimental procedure, and data analysis.