Titlebook: Agrobacterium Protocols; Volume 2 Kan Wang Book 2015Latest edition Springer Science+Business Media New York 2015 Agrobacterium.Agrobacteriu

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Kan WangIncludes cutting-edge methods and protocols.Provides step-by-step detail essential for reproducible results.Contains key notes and implementation advice from the experts

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Methods in Molecular Biologyhttp://image.papertrans.cn/a/image/151689.jpg

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Agrobacterium Protocols978-1-4939-1658-0Series ISSN 1064-3745 Series E-ISSN 1940-6029

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Philippe Major,Milan Matthiesenformation: tetraploidy, chimeras, and escape. Using a liquid culture system for somatic embryogenesis, Akasaka-Kenedy et al. (Plant Sci 166: 763–769, 2004) overcame these problems and established an efficient transformation system; the protocol introduced in this chapter is based on this method.

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1064-3745 ation advice from the experts.Rapid changes and significant progress have been made in the .Agrobacterium. field, such as genetically transforming plants for both basic research purposes and agricultural development. In .Agrobacterium Protocols, Third Edition, Volumes 1 .and. 2., a team of leading e

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Dao Companion to Korean Confucian Philosophy cocultivation medium; selection and regeneration of transformed shoots are carried out on selection medium containing 50 mg/L kanamycin (Km) and 250 mg/L timentin (or cefotaxime) for 3–5 months. In this protocol, the optimal media for shoot proliferation and shoot regeneration from leaf explants are genotype dependent.

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Cherry cocultivation medium; selection and regeneration of transformed shoots are carried out on selection medium containing 50 mg/L kanamycin (Km) and 250 mg/L timentin (or cefotaxime) for 3–5 months. In this protocol, the optimal media for shoot proliferation and shoot regeneration from leaf explants are genotype dependent.

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Melon (,)formation: tetraploidy, chimeras, and escape. Using a liquid culture system for somatic embryogenesis, Akasaka-Kenedy et al. (Plant Sci 166: 763–769, 2004) overcame these problems and established an efficient transformation system; the protocol introduced in this chapter is based on this method.