Titlebook: Affinity Chromatography; Methods and Protocol Michael Zachariou (Director Project Management) Book 2008Latest edition Humana Press 2008 Chr

Cabinet

喃喃訴苦

Rationally Designed Ligands for Use in Affinity Chromatography of antibodies. The concepts of rational design and solid-phase combinatorial chemistry were used for the discovery of a synthetic PpL mimic affinity ligand. The procedure presented in this chapter represents a general approach with the potential to be applied to different systems and target proteins.

BLANC

Preparation, Analysis and Use of an Affinity Adsorbent for the Purification of GST Fusion Protein protein displays a dissociation constant of 0.6 x10. M to glutathione immobilized to Streamline?. Ligand density optimization, fusion protein elution conditions (pH and glutathione concentration) and ligand orientation are briefly discussed.

責(zé)任

Corporate Venturing in der Praxisthentic epitope mimic and coupling the peptide mimotope to an affinity resin for purifying antibodies from human serum. There are several other applications of phage peptides that could be used for affinity chromatography; the approaches are outlined, but detailed methods have not been included.

丑惡

Das Unternehmen im Stakeholder-Dialoghich ligand is bound to it. Here, we describe a procedure for immobilization of HaloTag? fusion proteins and how immobilized proteins can be used to study protein–protein and protein–DNA interactions in vivo and in vitro.

Forsake

玩忽職守

The Use of TAGZyme for the Efficient Removal of N-Terminal His-Tags The UZ-HT15 tag and the method can be applied to virtually any protein. A description of the cloning strategy for the design of the genetic construction, two alternative approaches and a simple test to assess the performance of the tag removal process are also included.

繁榮地區(qū)

侵略

Immobilized Metal Ion Affinity Chromatography of Native Proteinsm, that of the interaction between histidine and immobilized metal ions, those that enable purification of proteins that lack surface histidines and the operation of IMAC supports in cation exchange mode.

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