Titlebook: Affinity Chromatography; Methods and Protocol Michael Zachariou (Director Project Management) Book 2008Latest edition Humana Press 2008 Chr

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書目名稱Affinity Chromatography影響因子(影響力)




書目名稱Affinity Chromatography影響因子(影響力)學(xué)科排名




書目名稱Affinity Chromatography網(wǎng)絡(luò)公開度




書目名稱Affinity Chromatography網(wǎng)絡(luò)公開度學(xué)科排名




書目名稱Affinity Chromatography被引頻次




書目名稱Affinity Chromatography被引頻次學(xué)科排名




書目名稱Affinity Chromatography年度引用




書目名稱Affinity Chromatography年度引用學(xué)科排名




書目名稱Affinity Chromatography讀者反饋




書目名稱Affinity Chromatography讀者反饋學(xué)科排名

Monotonous

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Affinity Precipitation of Proteins Using Metal ChelatesThe copolymers of vinylimidazole with .-isopropylacrylamide are easily synthesized by radical polymerization. When loaded with Cu(II) and Ni(II) ions, these copolymers are capable of selectively precipitating proteins with natural metal-binding groups or histidine-tagged recombinant proteins.

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Dye Ligand Chromatographyanalogs, offering affinity interactions with their corresponding enzymes. This chapter describes a dye ligand chromatography technique for purifying proteins from overexpression, in mammalian cell culture. The method begins with batch binding in order to rapidly select binding and elution conditions

Entreaty

Purification of Proteins Using Displacement Chromatographyurified components at high concentrations, less tailing during elution, high throughput and high resolution. Displacer affinity and its utilization are the critical components of displacement chromatography. Particularly, the nonspecific interactions between the displacer and the stationary phase ca

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NATTY

Phage Display of Peptides in Ligand Selection for Use in Affinity Chromatographyghtforward process involving several cycles of selection against target molecules, and the resulting ligands can be tailored to various applications. In this chapter we describe detailed methods to select peptide ligands for affinity chromatography, with particular focus on selection of peptides tha

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Preparation, Analysis and Use of an Affinity Adsorbent for the Purification of GST Fusion Proteine (GST) fusion protein in packed and expanded bed chromatographic processes. The protein is composed of GST fused to a zinc finger transcription factor (ZnF). Glutathione, the affinity ligand for GST purification, is covalently immobilized to a solid-phase adsorbent (Streamline?). The GST–ZnF fusion

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Immobilized Metal Ion Affinity Chromatography of Histidine-Tagged Fusion Proteinspression vectors for the production of histidine-tagged recombinant proteins as well as the variety of stationary supports for their separation Make IMAC an attractive and versatile choice for fast and reliable protein purification. It is not uncommon for IMAC purification to yield near homogenous t