Titlebook: Adenovirus Methods and Protocols; Volume 1: Adenovirus William S. M. Wold,Ann E. Tollefson Book 2007Latest edition Humana Press 2007 DNA.EL

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Zahldarstellungen und Stellenwertsysteme,ailed method for the rapid construction of adenoviral vectors. The method described here uses homologous recombination machinery of . BJ5183 to construct plasmids used in generation of adenoviral vectors. With this method, no ligation steps are involved in generating the plasmids, and any region of

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Fazit zu den wichtigsten Steuerarten,enovirus (HAdV) and the promiscuous nature of the virus have made the use of nonhuman adenoviruses an attractive alternative. Moreover, readministration of viral vectors is often required to maintain therapeutic levels of transgene expression, resulting in vector-specific immune responses. Although

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https://doi.org/10.1007/978-3-8348-9122-8ll types used to propagate the more commonly used subgroup C serotypes (Ad2 and Ad5) and there is no standard plaque assay. Methods to propagate Ad40 in complementing cell lines and to evaluate infectivity and particle number are presented in this chapter.

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,Einfüuhrung in die Systemtheorie,le. In this chapter we describe an immunocompetent, semi-permissive cotton rat tumor model to evaluate the safety and efficacy of oncolytic Ad vectors. With this model one can investigate the effect of the host immune system on the vector-tumor interaction as well as the vector’s effect on normal ho