Titlebook: Adenovirus Methods and Protocols; Volume 1: Adenovirus William S. M. Wold,Ann E. Tollefson Book 2007Latest edition Humana Press 2007 DNA.EL

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William S. M. Wold,Ann E. TollefsonDetailed description of quality control testing of vectors.Methods for determination of virus titers.Sensitive assays for testing viral DNA presence in samples.Developing new, permissive immunocompete

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Adenovirus Methods and Protocols978-1-59745-166-6Series ISSN 1543-1894 Series E-ISSN 1940-6037

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https://doi.org/10.1007/978-3-8348-9122-8ll types used to propagate the more commonly used subgroup C serotypes (Ad2 and Ad5) and there is no standard plaque assay. Methods to propagate Ad40 in complementing cell lines and to evaluate infectivity and particle number are presented in this chapter.

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https://doi.org/10.57088/978-3-7329-8939-3 used to quantitate adenoviruses (such as the plaque assay or fluorescent focus assay) are time-consuming and subjective in their interpretation. Here we describe a flow cytometric method that eliminates these disadvantages and provides a quantitative and reliable method of focus-forming unit (FFU) assay.

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Kristina Reiss,Gerald SchmiederDNA damage, and apoptosis. E4 is essential for viral growth in most cell lines. In this chapter, the current knowledge of the functions of six E4 products is summarized briefly. Protocols are presented for manipulation of E4, incorporation of E4 mutations into the viral genome, and growth of E4 muta

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Anwendungen der elementaren Zahlentheorie, deletion mutant. In general, the deletion mutant then must be separated physically from the helper for use in subsequent experiments. This chapter includes suggestions for selection of helper viruses, protocols for the production of stocks by complementation, and procedures for physical separation