Titlebook: West Nile Virus; Methods and Protocol Tonya M. Colpitts Book 2016 Springer Science+Business Media New York 2016 West Nile Virus infection.i

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Methodology for Identifying Host Factors Involved in West Nile Virus Infection,rplay of the virus with several host factors. Identification of the host factors impacting on WNV infection can enhance our understanding of virus infection mechanisms, host immune defense mechanisms, and also reveal novel host targets that can be developed as antivirals. RNA interference (RNAi) is

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ELISA and Neutralization Methods to Measure Anti-West Nile Virus Antibody Responses,y investigations to assess immunogenicity of candidate vaccines or to evaluate seroconversion following challenge with WNV. Here, we outline the steps for screening or titrating of total antibodies by indirect enzyme linked immunosorbent assay (ELISA) as well as assessment of neutralizing antibody t

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Safe Handling of West Nile Virus in the Insectary,ew case reports of individuals infected with members of the Flaviviridae while performing biomedical research in traditional laboratories and insectaries. We highlight the most common transmission route and summarize recommendations for facilities and protocols designed to prevent laboratory-acquire

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Techniques for Experimental Infection of Mosquitoes with West Nile Virus,e susceptible to WNV infection. WNV infection in humans can result in fever, meningitis, and encephalitis. Approved human vaccines or therapies are not available for WNV infection. In this chapter, we focus on the techniques for WNV infection and detection in mosquitoes. The technical details includ

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Detection Protocols for West Nile Virus in Mosquitoes, Birds, and Nonhuman Mammals,medially important mosquito-borne pathogen is an important part of public health practice. Here we present protocols for testing environmental samples such as mosquitoes, nonvertebrate mammals, and birds for this virus, including RT-PCR, virus isolation in cell culture, and antigenic assays, as well

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An Overview of Current Approaches Toward the Treatment and Prevention of West Nile Virus Infection,s for an urgent need of effective treatments and prevention measures. Although the licensing of several WNV vaccines for veterinary use provides a proof of concept, similar efforts on the development of an effective vaccine for humans remain still unsuccessful. Increased understanding of biology and