Titlebook: West Nile Virus; Methods and Protocol Tonya M. Colpitts Book 2016 Springer Science+Business Media New York 2016 West Nile Virus infection.i

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Field Surveillance Methods for West Nile Virus,th posed by naturally circulating WNV. In this chapter we focus on methods for detection of both WNV and its enzootic transmission. Methods for virus detection include CDC mini light trap, CDC gravid trap, and dead bird surveillance. Methods for transmission detection include passive box traps, chicken-baited traps, and sentinel chickens.

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Field Methods and Sample Collection Techniques for the Surveillance of West Nile Virus in Avian Hoscies to species thus surveillance efforts can focus both on birds that survive infection and those that succumb. Here we describe methods for the collection and sampling of live birds for WNV antibodies or viremia, and methods for the sampling of dead birds. Target species and study design considerations are discussed.

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Statistical Tools for the Interpretation of Enzootic West Nile virus Transmission Dynamics,lth officials to prescribe effective WNV management tactics. Stepwise procedures for the calculation of mosquito infection rates (IR) and vector index (VI) are presented alongside statistical tools that require additional computation. A brief review of advantages and important considerations for each statistic’s use is provided.

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Generating West Nile Virus from an Infectious Clone,template for in vitro transcription to synthesize infectious RNA. The resulting RNA is transfected into cells and after several days WNV is recovered in the culture supernatant. This method can be used to generate virus from infectious clones encoding high- and low-pathogenicity strains of WNV, as well as chimeric virues.