Titlebook: Biological Aging; Methods and Protocol Trygve O. Tollefsbol Book 2013Latest edition Springer Science+Business Media, New York 2013 Biologic

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https://doi.org/10.1007/978-3-642-47596-2 using animal models. In this chapter, we focus on candidate CRMs including sirtuin-activating compounds (STACs) and mTOR inhibitors, their slowing of aging, and methods for evaluation of lifespan and metabolic disorders.

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Uwe Steffens,Ankatrin La?-Lentzschoach in .. A mutant selected from such a population is stabilized to recover a pure homozygous strain. In this chapter, we categorize the protocol into mutagenesis, phenotype screen, and outcross and provide time-tested procedures for their implementation to create long-lived worm mutants.

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Mitochondria-Targeted Antiaging Gene Therapy with Adeno-associated Viral Vectors,we outline strategies on the generation of an AAV vector expressing the mitochondria-targeted catalase gene (AV.RSV.MCAT). We also describe methods for evaluating physiological impact of AV.RSV.MCAT on muscle contractility and running performance in mice.

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