Titlebook: Biological Aging; Methods and Protocol Trygve O. Tollefsbol Book 2013Latest edition Springer Science+Business Media, New York 2013 Biologic

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978-1-4939-6313-3Springer Science+Business Media, New York 2013

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Trygve O. TollefsbolFocuses on contemporary approaches and established protocols to study aging cell culture.Provides step-by-step detail essential for reproducible results.Contains key notes and implementation advice fr

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Aging Studies in Drosophila Melanogaster,describe methods commonly used in . aging research. These include basic approaches for preparation of diets and measurements of lifespan, food intake, and reproductive output. We also describe some commonly used assays to measure changes in physiological and behavioral functions of . in aging, such as stress resistance and locomotor activity.

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Methods of Testing Pharmacological Drugs Effects on Aging and Life Span in Mice, includes the selection of mouse strain, sex, age at start of treatment, housing conditions, design of the long-term study, some noninvasive methods of assessment of biomarkers of aging, life-span parameters, pathology examination, and statistical treatment of the results.

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Uwe Steffens,Ankatrin La?-Lentzsch separation of cells based on different characteristics which can be measured by flow cytometry. Here, we describe various methods by which senescent cells can be sorted from mixed cultures and discuss how different methods impact on the posterior analysis of sorted populations.