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Titlebook: Viroids; Methods and Protocol Ayala L. N. Rao,Irene Lavagi-Craddock,Georgios Vid Book 2022 Springer Science+Business Media, LLC, part of Sp

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書目名稱Viroids
副標題Methods and Protocol
編輯Ayala L. N. Rao,Irene Lavagi-Craddock,Georgios Vid
視頻videohttp://file.papertrans.cn/984/983282/983282.mp4
概述Includes cutting-edge methods and protocols.Provides step-by-step detail essential for reproducible results.Contains key notes and implementation advice from the experts
叢書名稱Methods in Molecular Biology
圖書封面Titlebook: Viroids; Methods and Protocol Ayala L. N. Rao,Irene Lavagi-Craddock,Georgios Vid Book 2022 Springer Science+Business Media, LLC, part of Sp
描述.This volume explores the latest methods used by researchers to study the detection, characterization, and various aspects of viroids. The chapters in this book are organized into seven parts and cover topics such as detection methods based on the biology of viroids; detection techniques based on electrophoresis and hybridization techniques; PCR-based techniques that provide high degrees of sensitivity; emerging area of nucleic acid sequence-based technology; and emerging techniques in viroid research such as RNA silencing, splicing, and viroid structure. Written in the highly successful .Methods in Molecular Biology. series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.?.Cutting-edge and comprehensive, .Viroids: Methods and Protocols. is a valuable resource for researchers and graduate students who are working with viroid diseases.?.
出版日期Book 2022
關鍵詞sPAGE gel electrophoresis; Polyprobes; RT-PCR; Multiplex RT-PCR; Next-generation sequencing
版次1
doihttps://doi.org/10.1007/978-1-0716-1464-8
isbn_softcover978-1-0716-1466-2
isbn_ebook978-1-0716-1464-8Series ISSN 1064-3745 Series E-ISSN 1940-6029
issn_series 1064-3745
copyrightSpringer Science+Business Media, LLC, part of Springer Nature 2022
The information of publication is updating

書目名稱Viroids影響因子(影響力)




書目名稱Viroids影響因子(影響力)學科排名




書目名稱Viroids網(wǎng)絡公開度




書目名稱Viroids網(wǎng)絡公開度學科排名




書目名稱Viroids被引頻次




書目名稱Viroids被引頻次學科排名




書目名稱Viroids年度引用




書目名稱Viroids年度引用學科排名




書目名稱Viroids讀者反饋




書目名稱Viroids讀者反饋學科排名




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Detection of Viroids Using RT-qPCRptase generates a cDNA copy of a portion of the target RNA molecule. After first-strand cDNA synthesis, RNA template from cDNA:RNA hybrid molecule is removed by digestion with RNase H to improve the sensitivity of PCR step. This cDNA is then be used as a template for amplification of viroid sequence in PCR.
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