Titlebook: Viral Applications of Green Fluorescent Protein; Methods and Protocol Barry W. Hicks Book 2009 The Editor(s) (if applicable) and The Author

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The Use of Green Fluorescent Fusion Proteins to Monitor Herpes Simplex Virus Replication,nfected cell. Normally, the replication proteins accumulate within replication compartments, which expand as viral DNA synthesis increases. If viral replication is blocked, either by the addition of drugs or a genetic lesion, prereplicative sites are observed. Observing the distribution of a GFP-tag

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Modified Bovine Herpesvirus 1 for Protein Secretion,er proteins, antigens, or immunomodulatory active molecules was (and still is) the expression of the protein of interest from an entire gene consisting of promoter, 5′- and 3′-noncoding regions, the open reading frame (ORF), and a signal sequence for polyadenylation. This approach is doubtlessly app

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Book 2009d versatile tool in scientific research. In .Viral Applications of Green Fluorescent Protein: Methods and Protocols., leading investigators from around the world contribute detailed examples of both the construction and application of fluorescent proteins delivered by viruses in a format crafted to

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Adenovirus-Mediated Delivery of Short Hairpin RNA (shRNA) Mediates Efficient Gene Silencing in Termenoviruses and their application to adult cardiac myocytes, which represent a standard experimental model in research related to cardiac physiology and pathophysiology. Feasibility of this approach is demonstrated by effective ablation (>80%) of both, a transgene encoding for eGFP and the endogenous muscarinic M. acetylcholine receptor.