Titlebook: Vertebrate Embryogenesis; Embryological, Cellu Francisco J. Pelegri Book 2011 Springer Science+Business Media, LLC 2011 TILLING.ZFN-mediate

alliance

Harrowing

Gene Targeting in the Mouse, variety of biological research areas. More recently, the original knock-out technology has been further refined to develop advanced strategies to generate conditional knock-out and knock-in mice. In this chapter, an overview of gene-targeting strategies is presented and procedures to generate these genetically engineered mice are discussed.

NAVEN

Manipulation of Gene Function in ,,mRNAs, respectively, into the early embryo. Herein we offer detailed protocols describing how to design and perform these experiments successfully, as well as a brief discussion of considerations for performing a microarray analysis in this organism.

phlegm

collagen

Host142

, Genetics,ods for genetic analysis of ., including protocols for controlled crosses both by natural spawning and by the surgical isolation of gametes; the identification and propagation of mutant lines; and strategies for positional cloning.

harbinger

Reptile Embryology,stological techniques (whole-mount histochemistry and immunohistochemistry). In addition, we provide a summary of basic reptilian husbandry requirements and discuss important details of embryonic nutrition, egg anatomy and sex determination.

Accessible

Antigen

Manipulating and Imaging the Early , Embryo,cellular levels. The year-round availability of easily fertilized eggs, the embryo’s large size and rapid development, and the hardiness of both adults and offspring against a wide range of laboratory conditions provide unmatched advantages for a variety of approaches, particularly “cutting and past

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