Titlebook: Vasculogenic Mimicry; Methods and Protocol Emily Marques dos Reis,Fernanda Berti Book 2022 Springer Science+Business Media, LLC, part of Sp

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Co-immunoprecipitation of Protein Complexes from Different Subcellular Compartments in Vasculogenic llular interactome of VE-cadherin and other key proteins involved in VM shed light to novel functions of endothelial proteins aberrantly expressed in tumor cells and their consequences in cell plasticity during VM development.

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Patient-Derived Xenograft Models for Studying Vascular Mimicry in Melanoma In Vivocribes the patient-derived xenograft (PDX) models for studying vascular mimicry in melanoma in vivo. The chapter discusses methods for tissue collection, preimplantation processing, implantation, harvest, storage, and postharvest. Detection of vasculogenic mimicry has been described using immunohistochemical expression of markers.

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Immunohistochemistry for VM Markersany markers are debated if belong to the angiogenic process or VM exclusively, the immunohistochemistry of CD31 and the PAS reaction often clarify in frozen or paraffin sections the pathologic status and the vasculature grade of a tumor mass.

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Histological Evaluation of Long-Term Collagen Type I Culturede the protocols for periodic acid–Schiff (PAS) staining to evidence glycoproteins and CD31 immunohistochemistry to exclude the presence of this endothelial marker, as per definition by vasculogenic mimicry concept. This method allows to consider long-term culture as tissue, promoting the deeper study of vascular-like structures ..

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Histochemical Staining of Vasculogenic Mimicryaining Methods and Protocols in Vasculogenic Mimicry.” Here, we provide most up-to-date and detailed information upon microscopy and histology protocols for the identification and understanding of VM process in both in vitro and in vivo.