Titlebook: Vascular Tissue Engineering; Methods and Protocol Feng Zhao,Kam W. Leong Book 2022 The Editor(s) (if applicable) and The Author(s), under e

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Rabbit Surgery Protocol for End-to-End and End-to-Side Vascular Graft Anastomosis,lative larger caliber for anastomotic implantation of vascular grafts as preclinical testing before conducting large animal studies. Rabbits have similar hemostatic mechanism with human and can accommodate vascular grafts with various diameters at different locations, and thus provide a valid model

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Vascular Imaging in Small Animals Using Clinical Ultrasound Scanners,d (>30?MHz) has been a powerful investigative device for preclinical studies such as cardiovascular research. In this chapter, we describe the use of conventional ultrasound with a 2.5–10?MHz transducer as an investigative device for the measurement/detection of blood flow in rabbit model. The chapt

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Vascular Graft Implantation Using a Bilateral End-to-Side Aortoiliac Preclinical Model,ascular grafts are commonly done as end-to-end grafts in small animal models. Here we detail bilateral end-to-side aortoiliac graft implantation, which requires open surgery and the creation of vascular anastomoses between the graft material and the infrarenal aorta and iliac artery in a nonhuman pr

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Quantification of In Vitro Blood-Brain Barrier Permeability,erve as a valid substitute, the in vitro BBB should have the similar barrier function as that in vivo. This chapter summarizes the detailed methods for quantifying the barrier function, e.g., the permeability of the BBB to water, ions, and solutes for an in vitro BBB generated on the Transwell filte