Titlebook: Vascular Biology Protocols; Nair Sreejayan,Jun Ren Book 2007 Humana Press 2007 cardiovascular.endothelium.hypertension.migration.smooth mu

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Immunostaining of Mouse Atherosclerotic Lesions,aining is now a commonly applied technique to identify these numerous cell types and regulators in lesions. The principle of the technique is that an antibody is incubated with the tissue under conditions that favor a specific interaction with its antigen. This is subsequently visualized most common

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Animal Models of Hypertension,tic options for humans with hypertension, the molecular mechanisms underlying hypertension are still relatively unknown. The generation of hypertensive animal models provides an excellent modality to not only study the pathophysiology but also test innovative therapeutics. This chapter describes the

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Rat Models of Cardiac Insulin Resistance,d diabetes can also lead to renal failure, blindness, heart attack, stroke, and amputation. The focus of this chapter will be to review the different mechanisms of insulin resistance (IR)/type 2 diabetes and various animal models used to study cardiac changes during these conditions.

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The Isolated, Perfused Pseudo-Working Heart Model, use of cardiovascular models. Unfortunately, many molecular or cell-based screens are not perfect predictors of activity in vivo. One rapid means to test “proof of principle” in hearts is the isolated or Langendorff perfused heart system. This allows direct measurement of cardiac contractile functi

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Detection and Quantification of Apoptosis in the Vasculature,y of cell death in the vasculature has significantly contributed to our knowledge of vascular disease pathology and has played a role in identifying potential therapeutic strategies for these diseases. This chapter describes a number of standard, widely used protocols for detecting and quantifying a

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