Titlebook: VEGF Signaling; Methods and Protocol Lorna Fiedler Book 2015 Springer Science+Business Media New York 2015 Cardiovascular Development.Lymph

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Detection and Quantification of Vascular Endothelial Growth Factor Receptor Tyrosine Kinases in Prim VEGF levels. Here, we present a robust immunoblot-based protocol for detecting and quantifying VEGFRs in human endothelial cells. Using internal and external standards, we can rapidly evaluate receptor copy number and assess how this is altered in response to the cellular environment.

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Analysis of VEGF-Mediated ERK5 Activity in Endothelial Cellsta. Phos-tag? Acrylamide is a reagent that enables specific tagging of phosphorylated proteins, resulting in retarded mobility and a distinct upward band shift from the non-phosphorylated protein following SDS-PAGE. Here, we describe the details of Phosphate affinity SDS-PAGE of ERK5 using acrylamide-pendant Phos-tag?.

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VEGF Gene Transfer to the Utero-Placental Circulation of Pregnant Guinea Pigs to Enhance Fetal Growt viruses with the host tissue. Our studies have shown that adenoviral gene delivery using pluronic gel resulted in the highest transduction efficiency compared to intra-arterial administration or external administration in PBS as a vehicle, when measured by X-gal staining, immunohistochemistry, or Western blotting.