| 書目名稱 | Tissue Microarrays | | 副標題 | Methods and Protocol | | 編輯 | Ronald Simon | | 視頻video | http://file.papertrans.cn/926/925679/925679.mp4 | | 概述 | Expert TMA protocols for all types of tissues including formalin fixed tissues, frozen tissues, and cell suspensions.Comprehensive description of in-situ techniques at the DNA, RNA, and protein level. | | 叢書名稱 | Methods in Molecular Biology | | 圖書封面 |  | | 描述 | The tissue microarray (TMA) method presents as a modern high technology, although its roots go back to the 80s when researchers frst started to combine several small pieces of tissues into so-called sausage blocks. In this respect, the TMA invention was not frstly characterized by technical improvements, but its true novelty was to link clinical data to the tissues that were combined on one slide. The very high number of tissues that can be included into one TMA, the small size and regular shape of the tissue spots, the preser- tion of integrity of the donor tissue blocks, and the highly organized array pattern that allows for reliable allocation of clinical data to individual tissue spots made it a discrete technique with unique features. When the TMA technology was developed 12 years ago, its beneft was controversially debated. While many researchers welcomed the method enthusiastically, there were c- cerns by others that results obtained from the small tissue cores used for TMA making would not be suffciently representative of the donor tissues. Meanwhile, the increasing use of this technology has imposingly demonstrated its tremendous utility in research. In fact, basically all | | 出版日期 | Book 2010 | | 關鍵詞 | DNA; Microarray; Prevalence array; Prognosis array; Progression array; RNA; TMA technology; genes; hybridiza | | 版次 | 1 | | doi | https://doi.org/10.1007/978-1-60761-806-5 | | isbn_softcover | 978-1-4939-6103-0 | | isbn_ebook | 978-1-60761-806-5Series ISSN 1064-3745 Series E-ISSN 1940-6029 | | issn_series | 1064-3745 | | copyright | Springer Science + Business Media, LLC 2010 |
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