| 書目名稱 | The Ubiquitin Code | | 編輯 | Manuel S Rodriguez,Rosa Barrio | | 視頻video | http://file.papertrans.cn/922/921601/921601.mp4 | | 概述 | Includes cutting-edge methods and protocols.Provides step-by-step detail essential for reproducible results.Contains key notes and implementation advice from the experts | | 叢書名稱 | Methods in Molecular Biology | | 圖書封面 |  | | 描述 | .This volume details novel and popular approaches to decipher the?.Ubiquitin Code.. Chapters guide readers through ubiquitin chain complexity,?methods to generate non-hydrolysable ubiquitin-NEDD8,?a?fully synthetic method for the linear synthesis of fluorescently labelled rhodamine LC3A and LC3B,?a?HECT?ligase-mediated ubiquitylation?.in vitro,?a?.RNAi-based screening?to identify DUBs,?a?Yuh1 activity?assay,?a pull-down to isolate?K48 and K63 chains?using?specific nanobodies,?identification of NeDDylated protein based in a?NEDD8.R74K?.mutant,?.in vivo.?identification?of NEDDylated proteins from?liver diseases,?UbL-ID method to identify?ubiquitin-like modified?proteins,?a LC3/GABARAP capturing system, and?methods to isolate peptides that are closely associated with proteasomes or trapped inside the core particle of proteasomes in eukaryotic cells,?among others.?Written in the successful?.Methods in Molecular Biology.?series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls...?..Authoritative and cutting-edge,?.The Ubiq | | 出版日期 | Book 2023 | | 關(guān)鍵詞 | label free proteomics; SILAC; Ubiquitin traps; SUMO antibodies; Gly-Gly antibodies | | 版次 | 1 | | doi | https://doi.org/10.1007/978-1-0716-2859-1 | | isbn_softcover | 978-1-0716-2861-4 | | isbn_ebook | 978-1-0716-2859-1Series ISSN 1064-3745 Series E-ISSN 1940-6029 | | issn_series | 1064-3745 | | copyright | The Editor(s) (if applicable) and The Author(s), under exclusive license to Springer Science+Busines |
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