Titlebook: Synthetic Promoters; Methods and Protocol Mario Andrea Marchisio Book 2024 The Editor(s) (if applicable) and The Author(s), under exclusive

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maladorit

idiopathic

Promoters in ,: A Toolbox for Fine-Tuned Gene Expressionral inducible, derepressed, and constitutive promoters, as well as engineered synthetic/hybrid promoters, orthologous promoters from related yeasts, and emerging bidirectional promoters. Key examples, characteristics, and regulatory mechanisms are discussed for each promoter class. Recent efforts in

Collision

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FLORA

perpetual

Simultaneous Plate-Reader Characterization of Promoter Activity and Cell Growth in Engineered Mammalization of both suspension and adherent cell lines when these are grown under different environmental conditions and treated with chemotherapeutic drugs. The method also supports characterization of stably engineered cell lines and identification of desired promoters based on fluorescence output.

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VICT

opalescence

TED: Enhancing Translation Efficiency in Bacterial Expression Systemside sequence into the 5′ untranslated region between the promoter and the Shine–Dalgarno (SD) sequence. Various expression levels can be observed depending on the inserted sequence and its length, even with an identical promoter.