Titlebook: Synthetic DNA; Methods and Protocol Randall A. Hughes Book 2017 Springer Science+Business Media, LLC, part of Springer Nature 2017 syntheti

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Simultaneous Removal of Multiple DNA Segments by Polymerase Chain Reactionsce of certain restriction enzyme recognition sites. Recent development of restriction-free cloning approaches has greatly enhanced the flexibility and speed of molecular cloning. Most restriction-free cloning methods focus on DNA assembly. Much less work has been dedicated towards DNA removal. Here

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Rapid Construction of Recombinant Plasmids by QuickStep-Cloninglasmid. It was designed specifically to address the major drawbacks of previously reported cloning methods. The fully optimized protocol allows for a seamless integration of a long DNA fragment into any position within a plasmid of choice, in a time-efficient and cost-effective manner, without the n

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Book 2017eadily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls...?.Authoritative and cutting-edge, .Synthetic DNA: Methods and Protocols. aims to help researchers further their research on manipulate DNA sequences..

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Shuffle Optimizer: A Program to Optimize DNA Shuffling for Protein Engineeringthe sheared fragments and recover and clone the library. The Shuffle Optimizer program and these protocols will be useful to anyone desiring to perform any of the nucleotide homology-dependent shuffling methods.

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