Titlebook: Stem Cells and Aging; Methods and Protocol Kursad Turksen Book 2019Latest edition Springer Science+Business Media, LLC, part of Springer Na

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Isolation and Culture of Individual Myofibers and Their Adjacent Muscle Stem Cells from Aged and Adse to their endogenous niche as possible. Here we describe the isolation, culture, transfection with siRNA, and subsequent immunostaining for muscle stem cells on their adjacent myofibers from aged and young mice.

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Adipogenic and Osteogenic Differentiation of In Vitro Aged Human Mesenchymal Stem Cells,the regenerative and therapeutic potential of MSCs. In this chapter, we describe a step-by-step protocol that aims to induce adipogenic and osteogenic differentiation of in vitro aged human MSCs and highlight noteworthy issues that may arise during the process.

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Human Synovium-Derived Mesenchymal Stem Cells: Ex Vivo Analysis, human synovium. This includes determination of the proportions of viable cells in ex vivo isolated fractions before the seeding of the cells and a description of how to carry out colony-forming fibroblast assays to quantify the clonogenicity of these cells.

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Metabolomic and Proteomic Analyses of Mouse Primordial Germ Cells,r the control of cell function and differentiation, little is known about the metabolic features of PGCs. Here, we describe use of an integrated metabolomic, proteomic, and energy metabolic analysis method to comprehensively elucidate the metabolic characteristics of PGCs using mass spectrometry.

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Book 2019Latest editionthodologies are well-established and described in easy to follow fashion so as to be valuable for not only experts but novices in the ever-developing stem cell field. Written for the highly successful .Methods in Molecular Biology. series, chapters include introductions to their respective topics, l

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Targeted, Amplicon-Based, Next-Generation Sequencing to Detect Age-Related Clonal Hematopoiesis,s atherosclerotic cardiovascular disease. Therefore, the detection of CHIP has important clinical consequences. Herein, we present a protocol for the generation of targeted, amplicon-based, NGS libraries for ion semiconductor sequencing and CHIP detection, using Ion Torrent platforms.

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Infrared Spectroscopy and Imaging in Stem Cells and Aging Research,ernative to get new information into the field of stem cell biology for chemical analysis, quantification, and imaging of stem cells. Infrared spectroscopy and imaging coupled with chemometric methods can be used as novel and complimentary methods to obtain new insight into stem cell studies for future therapeutic and regenerative medicine.

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