Titlebook: Stem Cell Technologies in Neuroscience; Amit K. Srivastava,Evan Y. Snyder,Yang D. Teng Book 2017 Springer Science+Business Media LLC 2017

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Isolation and Characterization of Extracellular Vesicles in Stem Cell-Related Studies,ght of strong interest in stem cell-based therapies, EVs are particularly attractive as therapeutic entities because they do not replicate and may have other safety advantages over whole stem cells. Here, we examine the current literature on stem cell-derived EVs, finding that differential centrifug

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,Three-Dimensional Cultures of Human Neural Stem Cells: An Application for Modeling Alzheimer’s Dised in current AD mouse models. In this chapter, we describe instructions and troubleshooting for establishing 3D human cell culture models that can be used to analyze β-amyloid and tau pathology in a dish.

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Schwann Cell Isolation and Culture Reveals the Plasticity of These Glia,ve rise to neurons as well as more Schwann cells, depending upon the signals they receive. Finally, we have previously demonstrated that Schwann cells expanded in vitro can be used to engraft a biological bridge between distant axonal stumps, promoting axonal regeneration when none would otherwise occur.

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Development of Mouse Cell-Based In Vitro Blood-Brain Barrier Models,ep-by-step protocol starting from the homogenization of brain tissue, digestion steps, seeding, and immortalization of the cells is given. Usually, it takes about 5 weeks to obtain a homogenous, immortalized BMEC line which can be used up to 50 passages.

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Standardized Cryopreservation of Stem Cells,method for controlled rate freezing and thawing of human stem cells that is reliable, inexpensive, and user friendly. This method successfully prepares stem cells for long-term cryogenic storage while ensuring maximal post-thaw cell viability.

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