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Titlebook: Stem Cell Mobilization; Methods and Protocol Gerd Klein,Patrick Wuchter Book 2019 Springer Science+Business Media, LLC, part of Springer Na

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發(fā)表于 2025-3-21 17:57:36 | 只看該作者 |倒序瀏覽 |閱讀模式
書目名稱Stem Cell Mobilization
副標題Methods and Protocol
編輯Gerd Klein,Patrick Wuchter
視頻videohttp://file.papertrans.cn/878/877203/877203.mp4
概述Includes cutting-edge techniques.Provides step-by-step detail essential for reproducible results.Contains key implementation advice from the experts
叢書名稱Methods in Molecular Biology
圖書封面Titlebook: Stem Cell Mobilization; Methods and Protocol Gerd Klein,Patrick Wuchter Book 2019 Springer Science+Business Media, LLC, part of Springer Na
描述This book provides detailed state-of-the-art protocols for analyzing individual aspects of stem cell mobilization at the molecular and cellular level with contributions encompassing the most recent technological developments. Specifically, the volume covers cell adhesion and migration assays in three-dimensional models, imaging techniques for the determination of blood flow or hypoxic status, innovative biomechanical studies of stem cells, and assays to determine the involvement of proteases or complement factors, as well as other key topics. Written for the highly successful .Methods in Molecular Biology. series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.?.Authoritative and practical, .Stem Cell Mobilization: Methods and Protocols. is an ideal guide for all laboratories in their endeavors to decipher the mechanisms of stem cell mobilization, ultimately helping stem cell donors and patients around the world..
出版日期Book 2019
關鍵詞Stem cell harvesting; Hematopoietic stem cells; Transplantation; Cell adhesion; Migration assays
版次1
doihttps://doi.org/10.1007/978-1-4939-9574-5
isbn_softcover978-1-4939-9576-9
isbn_ebook978-1-4939-9574-5Series ISSN 1064-3745 Series E-ISSN 1940-6029
issn_series 1064-3745
copyrightSpringer Science+Business Media, LLC, part of Springer Nature 2019
The information of publication is updating

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Eric Gottwald,Cordula Nies,Patrick Wuchter,Rainer Saffrich,Roman Truckenmüller,Stefan Giselbrecht
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Angela Jacobi,Philipp Rosendahl,Martin Kr?ter,Marta Urbanska,Maik Herbig,Jochen Guck
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In Vitro Dynamic Phenotyping for Testing Novel Mobilizing Agents,ilization agents. After extracting the contour of each cell, one can analyze the dynamics of cell “shapes” step-by-step, yielding various levels of information ranging from the principal mode of deformation, the persistence of deformation patterns, and the energy consumption by HSPC in the absence a
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A Microcavity Array-Based 3D Model System of the Hematopoietic Stem Cell Niche,s with a diameter of 300?μm and a depth of up to 300?μm and arranged in a 634-cavity array. The polymer films are provided with 3?μm pores and thus allow perfusion of the culture medium. The microcavity arrays can be inserted into a microbioreactor where a closed circulation loop can be tightly cont
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Migration Assay for Leukemic Cells in a 3D Matrix Toward a Chemoattractant,el system mimicking the extracellular matrix in vivo. The cells are encapsulated into the hydrogel network during polymerization, and a CXCL12 gradient is introduced in the enclosed chambers to trigger migration. Cell migration in the 3D network of the hydrogel is monitored by time-lapse microscopy.
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