Titlebook: Staphylococcus aureus; Methods and Protocol Kelly C. Rice Book 2021 Springer Science+Business Media, LLC, part of Springer Nature 2021 FRET

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Bone Marrow-Derived Macrophage Infection Assay,compared to that found in whole animals. To this end, macrophage infection assays have become a key technique for studying the molecular mechanisms by which bacteria interact with the host. Herein, this chapter describes both how to produce macrophages from mouse bone marrow and the subsequent infection assays.

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,Measuring Staphylococcal Promoter Activities Using a Codon-Optimized β-Galactosidase Reporter,y promoters, and use of small sample volumes. In this chapter, details are provided for both standard and high-throughput quantitative assays that we have routinely used for . transcriptional profiling.

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Quantitative Hemolysis Assays,d on agar. Furthermore, plate-based assays are primarily semiquantitative and often a more accurate determination of hemolytic potential is needed to discern differences between strains. Here, we describe a method to quantify hemolysin activity from broth or similarly grown cells.

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Book 2021cs, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls..Cutting-edge and authoritative, .Staphylococcus aureus: Methods and Protocols. is a valuable resource for anyone interested in this fascinating and developing field..